Anders Bergkvist - Plastocyanin
Plastocyanin
This was my original project, started in 1995. The main issue is to determine
a high-resolution structure of spinach plastocyanin. Due to several different
problems, this project has been somewhat delayed.
There are already several X-ray and NMR structures of plastocyanin from other
species; specifically, there are very nice NMR structures of parsley and french
bean. We therefore realized, a year or so into the project, that a structure of
spinach plastocyanin would not bring substantially new information to the field
and that we should expand our project to include NMR relaxation analyses as
well, before publication.
Later, our structure was also used in interaction studies between pc and cytf
and that will also be a part of my thesis work.
Publications in prepation:
"High-resolution Solution Structure and 15N-relaxation
Analysis of Spinach Plastocyanin", A Bergkvist,
S Young and B G Karlsson. Will be submitted to Biochemistry during spring of
2000.
"Side-chain Interactions in the Plastocyanin-Cytochrome f Complex
as Determined by 1H-NMR Chemical-shift Analysis",
M Ejdebäck, A Bergkvist, G W Canters, B G Karlsson and M Ubbink.
Will be submitted to Biochemistry this or next week.
"Side-chain Interactions in the E43Q/D44N and E59K/E60Q Plastocyanin
double-mutants to Cytochrome f Complex as Determined by
1H-NMR Chemical-shift Analysis", A Bergkvist,
M Ejdebäck, B G Karlsson and M Ubbink. Will be submitted during spring
of 2000.
Related publication:
"The structure of the complex of plastocyanin and cytochrome f,
determined by paramagnetic NMR and restrained rigid-body molecular
dynamics", M Ubbink, M Ejdebäck, B G Karlsson and D S Bendall,
Structure 6 (1998), 323-335
Last modified: November 17, 1999